Part:BBa_K4324101:Design
Xylitol Dehydrogenase from S. cerevisiae
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 606
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 263
Design Notes
The protein sequence was codon-optimised for expression in Escherichia coli K12 through the IDT codon optimisation tool as gBlocks® Gene Fragments, and ensured that EcoRI, XbaI, SpeI, and PstI restriction sites were not accidentally present within the CDS.
Source
This original protein sequence was taken from the XYL2 gene of Saccharomyces cerevisiae (also known as Baker's yeast) (strain ATCC 204508 / S288c), which can be found on the Uniprot database.
Alternatively, the protein sequence is pasted below:
MTDLTTQEAIVLERPGKITLTNVSIPKISDPNEVIIQIKATGICGSDIHYYTHGRIANYV
VESPMVLGHESSGIVALIGENVKTLKVGDRVALEPGIPDRFSPEMKEGRYNLDPNLKFAA
TPPFDGTLTKYYKTMKDFVYKLPDDVSFEEGALIEPLSVAIHANKLAKIKFGARCVVFGA
GPIGLLAGKVASVFGAADVVFVDLLENKLETARQFGATHIVNSGDLPHGVTVDSVIKKAI
GKKGADVVFECSGAEPCVRAGIEVCKAGGTIVQVGMGQEEIQFPISIIPTKELTFQGCFR
YCQGDYSDSIELVSSRKLSLKPFITHRYSFKDAVEAFEETSHHPLNNIKTIIEGPE
References
1. https://www.uniprot.org/uniprotkb/Q07993/entry
2. https://www.genome.jp/dbget-bin/www_bget?ec:1.1.1.9